Composite

Part:BBa_K2843002:Design

Designed by: Camille Lorie Michael de Figueiredo   Group: iGEM18_East_Chapel_Hill   (2018-10-09)


Fluoride RBS Mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 148
    Illegal XhoI site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the design of the sequence we afforded space for Xhol restriction cutting sites to allow for easy replacement and testing of riboswitches and promoters.



Source

The framework for the CHOP operon was developed by the East Chapel Hill 2017 team.

References